Preparation of Silymarin–quercetin Loaded Nanoparticles by Spontaneous Emulsification Solvent Diffusion Method Using D-alpha-tocopheryl Poly (Ethylene Glycol) 1000 Succinate

Silymarin, a flavonolignan, derived from Silybum marianum, family Asteraceae has long been used as hepatoprotective remedy. Silymarin cytoprotective activities due to its antioxidant property and free radical scavenging activity. The pharmacokinetic studies of past three decades revealed that silymarin poor absorption, rapid metabolism especially by Phase II ultimately oral bioavailability. Quercetin, flavonoid present in edible vegetables fruits, It is potent shows wide range biological functions. Quercetin improves blood levels efficacy number drugs since it P-Glycoprotein inhibitor also inhibits drug metabolizing enzymes. Both quercetin were, poorly soluble the water low advanced type formulation like polymeric nanoparticles (PNPs) can be successfully utilised for bioavailability enhancement targeting Silymarin-quercetin hepatocytes. A controlled release PNPs silymarin-quercetin were prepared spontaneous emulsification solvent diffusion (SESD) method using Poly Lactic-co-Glycolic Acid (PLGA) biodegradable polymer, D-alpha-tocopheryl poly (ethylene glycol) 1000 succinate (TPGS) solubilizer, an emulsifier. TPGS emulsifier further matrix material blended with PLGA was enhance encapsulation efficiency improve profile nanoparticles. Different formulations various drug: polymer ratios volume concentration surfactant, centrifugation time evaluated. effect parameters such drug/polymer ratio, surfactant content surface morphology size studied scanning electron microscopy (SEM) Transmission (TEM). Drug vitro profiles determined UV spectrophotometry. combination both this study spherical 100–200 nm. shown good producing hydrophobic improving loading Although amount had significant on nanoparticle morphology,